Journal: bioRxiv

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells

doi: 10.64898/2026.03.23.713743

Figure Lengend Snippet: (A) UMAP visualization of major cell clusters identified from single cell RNA sequencing (scRNA-seq) of NT2.5-LM breast-to-lung metastatic tumors after 3 weeks of treatment with: vehicle (V), Entinostat (E), anti-CTLA-4 + anti-PD-1 immune checkpoint inhibitors (PC), and Entinostat + anti-CTLA-4 + anti-PD-1 combination (EPC). Subclusters of MDSCs identified: G-MDSCs and M-MDSCs. (B) Iterative logistic regression analyses conducted on the major MDSC cluster comparing the Vehicle (V) and Entinostat + ICIs combination treatment (EPC). (C) Expression of Malat1 / MALAT1 in G- and M-MDSCs isolated from lung metastases of NT2.5-LM mice treated with vehicle vs. Entinostat for 3 weeks (left, n=2 from 5 pooled mice per treatment group), J774M murine MDSC-like cell line treated with Entinostat for 24 hours (middle, n=3), and human PBMC-derived MDSCs (hMDSCs) treated with Entinostat for 24 hours (right, n=3). (D) Median Fluorescence Intensity expression of Malat1 in CD45 + CD11b + MHC-II - F4/80 - Ly6G - Ly6C hi cells from lung metastases in 4T1 mice, treated with vehicle (V), Entinostat (E), and Entinostat + ICIs (EPC) for 3 weeks. (E) Expression of Malat1 in J774M cell line treated with various concentrations of various HDAC inhibitors, with corresponding targeted class and specific HDACs. One-way ANOVA for (C, E: all statistically significant with p<0.05, unless indicated as non-significant (ns)), Kruskal-Wallis test with Dunn’s correction for (D). * p<0.05, *** p< 0.001, **** p<0.0001. ENT = Entinostat; PAN = Panobinostat; BEL = Belinostat; VOR = Vorinostat; TSA = Trichostatin A; DOM = Domatinostat; CHL = Chlopynostat; PYR = Pyroxamide; SCA = Santacruzamate A; RGF = RGFP966; TMP = TMP269; SIS = SIS17.

Article Snippet: Immune checkpoint inhibitors (ICIs) used in this study: anti-CTLA-4 (BioXCell cat. #BE0131) and anti-PD-1 (BioXCell cat. #BE0146).

Techniques: Single Cell, RNA Sequencing, Expressing, Isolation, Derivative Assay, Fluorescence

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , RT-PCR of Notch1 - 4 transcripts in lung T reg and T eff cells isolated from PBS, OVA and OVA+UFP mouse groups (n=5). b , c , Flow cytometric analysis, cell frequencies and mean fluorescence intensity (MFI) of Notch4 expression on lung T reg and T eff cells in the respective treated groups (n=15). d , Flow cytometric analysis and cell frequencies of Notch4 expression on OT-II + CD4 + Foxp3 + T cells generated in co-cultures with sham or OVA 323–339 +UFP-pulsed alveolar macrophages without or with IL-6 or anti-IL-6R mAb (n=5). e , Flow cytometric analysis and cell frequencies of Notch4 + Helios – and Helios + lung T reg cells isolated from the respective treated groups (n=5). f , Flow cytometric analysis and cell frequencies of Notch4 expression on in vitro differentiated T reg cells derived from naive CD4 + T cells isolated from Foxp3 YFPCre , Foxp3 YFPCre Il6r Δ/Δ and Foxp3 YFPCre Stat3 Δ/Δ mice and either untreated or treated with IL-6 (n=6). g , ChIP assays for the binding of STAT3 and control (IgG) antibodies to the Notch4 promoter in lung T reg cells of OVA+UFP-treated Foxp3 YFPCre , and Foxp3 YFPCre Stat3 Δ/Δ mice (n=6). Each symbol represents one mouse. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis ( b,c,f ); two-way ANOVA with Sidak’s post hoc analysis ( a,d,e,g ). *P<0.05, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Fluorescence, Expressing, Generated, In Vitro, Derivative Assay, Binding Assay, Control

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a - c , Flow cytometric analysis, cell frequencies and (MFI) of Notch1, 2 and 3 expression on lung T reg and T eff cells in Foxp3 YFPCre (n=5). d , Cell frequencies of Notch4 expression on OT-II + CD4 + Foxp3 + T cells generated in co-cultures with sham or OVA 323–339 +UFP-pulsed alveolar macrophages without or with IL-1β, IL-25, IL-33, TSLP or TNF (n=5). e , ChIP assays for the binding of STAT3 and control (IgG) antibodies to the Notch1, 2 and 3 promoters in lung T reg cells of OVA+UFP-treated Foxp3 YFPCre , and Foxp3 YFPCre Stat3 Δ/Δ mice (n=5). Each symbol represents one mouse. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis ( a-c ); two-way ANOVA with Sidak’s post hoc analysis ( d,e ). **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Expressing, Generated, Binding Assay, Control

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , RT-PCR analysis of Notch4 expression in CD4 Cre mice in B-cells and T-cells (n=5). b , RT-PCR analysis of Notch4 expression in Foxp3 YFPCre mice in both T reg and T eff cells (n=5). c , d , IL-4 and IFN-γ expression in lung Foxp3 + CD4 + T reg . ( c ) and Foxp3 – CD4 + T eff cells. ( d ) derived from the respectively treated Foxp3 YFPCre , CD4 Cre Notch 4 Δ/Δ and Foxp3 YFPCre Notch 4 Δ/Δ mice (n=5). e , Airway hyperresponsiveness in Foxp3 YFPCre sensitized either with PBS or OVA, then challenged with OVA+UFP following transfer of OTII + Foxp3 YFPCre or OTII + Foxp3 YFPCre Notch4 Δ/Δ iT reg cells (n=5). f , Eosinophil numbers for the respective mouse groups (n=5). g , IL-4, IL-13, IL-17 and IFNγ expression in lung Foxp3 – CD4 – T eff cells. Each symbol represents one mouse (n=5). Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( a,c,d ); One-way ANOVA with Dunnett’s post hoc analysis ( e,f ). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Derivative Assay

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , Representative PAS-stained sections of lung tissues isolated from Foxp3 YFPCre , CD4 Cre Notch4 Δ/Δ or Foxp3 YFPCre Notch4 Δ/Δ mice segregated into PBS, OVA or OVA+UFP-treated groups (200X magnification). b , Inflammation scores in the respective lung tissues. c , AHR in the respective mouse groups in response to methacholine. ( d , e ) serum total and OVA-specific IgE concentrations. f , g , absolute numbers of lung CD4 + T cells and eosinophils. ( h , i ) IL-13 and IL-17 expression in lung Foxp3 + CD4 + T reg ( h) , and Foxp3 – CD4 + T eff cells. ( i ). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( b - i ). **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments. (White bars n=15), (black bars n=5) and (grey bars n=15).

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Staining, Isolation, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , scheme of the house dust mite (HDM) airway inflammation protocol. b , Representative PAS-stained sections of lung tissues isolated from Foxp3 YFPCre or Foxp3 YFPCre Notch4 Δ/Δ mice segregated into PBS, OVA or OVA+UFP-treated groups (200X magnification). c , Inflammation scores in the respective lung tissues. d , AHR in the respective mouse groups in response to methacholine. e , serum total IgE concentrations. ( f - h ), absolute numbers of lung CD4 + T cells, neutrophils and eosinophils. i , k , IL-4, IL-13, IL-17 and IFNγ expression in lung Foxp3 + CD4 + T reg ( i ) and Foxp3 – CD4 + T eff cells ( k ). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis (c - k ). **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments. n=5 mice per group.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Staining, Isolation, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , Scheme for the chronic airway inflammation mouse protocol b , Representative Sirius-Red-stained sections of lung tissues isolated from Foxp3 YFPCre or Foxp3 YFPCre Notch4 Δ/Δ mice segregated into PBS, OVA or OVA+UFP-treated groups (200X magnification). c , Collagen disposition measurement in the respective lung tissues. d , AHR in the respective mouse groups in response to methacholine. e , f , absolute numbers of lung CD4 + T cells and eosinophils. g , h , IL-4, IL-13, and IL-17 expression in lung Foxp3 + CD4 + T reg ( g ) and Foxp3 – CD4 + T eff cells ( h ). i , Serum OVA-specific IgE titers in the respective groups. Each symbol represents an independent sample. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( c-h). *P<0.05, ****P<0.0001. Data representative of two or three independent experiments. n=5 mice per group.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Staining, Isolation, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , Volcano plot of differential gene expression in Foxp3 YFPCre versus Foxp3 YFPCre Notch4 Δ/Δ T reg cells treated with OVA+UFP. FDR, false discovery rate; log2FC, log2(fold change). b , Enrichment pathway analysis of Hippo and Wnt pathways. c, Flow cytometric analysis and cell frequencies of Yap1 expression on lung T reg cells in the respective treated groups (n=5). d, Flow cytometric analysis and cell frequencies of β-Catenin expression on lung T reg cells in the respective treated groups (n=5). e, representative histogram, cell frequencies and MFI of phospho-Mob1 expression on lung T reg cells in the respective treated groups (light grey bar n= 4, dark grey bar n=4, light red bar n=5 and dark red bar n=4). f, representative histogram, cell frequencies and MFI of phospho-Lats1 T1079 expression on lung T reg cells in the respective treated groups (light grey bar n= 4, dark grey bar n=4, light red bar n=5 and dark red bar n=4). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( c,d ); one-way ANOVA with Dunnett’s post hoc analysis ( e,f ). ***P<0.001, ****P<0.0001.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Gene Expression, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , flow cytometric analysis and frequencies of IL13 + ILC2 (Lineage – T1/ST2 + cells) in mice of respective genotypes treated as indicated (n=5). b , c , In vitro suppression assays using ILC2 from OVA+UFP-treated Foxp3 YFPCre mice and lung T reg cells of the respective genotypes, treated as indicated (n=4) . d , GDF15 transcripts in T reg cells of Foxp3 YFPCre , Foxp3 YFPCre Notch4 Δ/Δ and Foxp3 YFPCre Ctnnb1 Δ/Δ (n=5). e , flow cytometric analysis and frequencies of GDF15 + lung T reg cells in the respective mouse genotypes treated as indicated (n=5). f , flow cytometric analysis and frequencies of IL-13 induced in naive ILC2 stimulated with IL-33, GDF15 or both (n=3). g , IL-13 expression in naive ILC2 incubated with Notch4 hi T reg cells from OVA+UFP treated mice without or with blocking GDF15 peptide (n=6). h , In vitro suppression assays using lung T reg cells and ILC2 isolated from OVA+UFP-treated Foxp3 YFPCre mice and incubated without or with GDF15 blocking peptide (n=4). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: two-way ANOVA with Sidak’s post hoc analysis ( a - e , h ); One-way ANOVA with Dunnett’s post hoc analysis ( f,g ). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: In Vitro, Expressing, Incubation, Blocking Assay, Isolation

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , d , Representative PAS-stained sections of lung tissues isolated from Foxp3 YFPCre and Foxp3 YFPCre Notch4 Δ/Δ with either PBS or OVA+UFP, the latter either alone or supplemented with GDF15 or GDF15 blocking peptide, as indicated (200X magnification), Inflammation score for the respective mouse groups (n=10). b , e , AHR in Foxp3 YFPCre and Foxp3 YFPCre Notch4 Δ/Δ treated as indicated (n=10). c , f , Frequencies and absolute numbers of ILC2, eosinophils, IL-4, and IL-13, expression in lung Foxp3 – CD4 + T eff cells in the respective groups (n=10) g, AHR in Rora Cre and Rora Cre Il4/Il13 Δ/Δ treated as indicated (n=5). h, Frequencies and absolute numbers of eosinophils, ILC2, IL-4, and IL-13, expression in lung Foxp3 – CD4 + T eff cells in the respective groups (n=5). Error bars indicate SEM. Statistical tests. One-way ANOVA with Dunnett’s post hoc analysis. ( a,c,d,f ), two-way ANOVA with Sidak’s post hoc analysis ( b , e , g , h ); *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Staining, Isolation, Blocking Assay, Expressing

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , b , Flow cytometric analysis, cell frequencies and MFI of Notch4 expression on circulating T reg cells ( a ) and T eff cells ( b ) of control and asthmatic subjects, the latter segregated for asthma severity (control: n=39; mild n=31; moderate: n=27; severe: n=11). c , flow cytometric analysis, cell frequencies and MFI of Notch4 expression on Helios + versus Helios – circulating T reg cells of control and asthmatic subjects (control: n=13; mild n=9, moderate n=14; severe: n=11). d , e , Flow cytometric analysis, cell frequencies and MFI of Yap ( d ) and β-catenin ( e ) expression on circulating T reg cells of control and severe asthmatic subjects (control n=24; mild n=15; moderate n=15; severe: n=11). f , Serum GDF15 concentrations in moderate and severe asthmatic subjects plotted as a function of Notch4 expression on circulating T reg cells (n=21). g , In vitro suppression third party CD4 + T cells (T eff ) by the Notch4 hi versus Notch4 lo T reg cells from severe asthmatics compared to T reg cells of control subjects (n=2 subjects, 3 replicates per dilution per subject). h , In vitro suppression assays of ILC2 activation using circulating Notch4 hi T reg cells of asthmatics subjects and control T reg cells of healthy controls, incubated at the indicated T reg cell:ILC2 ratios without or with GDF15 blocking peptide (n=5). i , Flow cytometric analysis of Notch4 expression in T reg cells of a healthy control and a severe asthmatic before and after treatment with anti-IL-6R mAb (n=1). Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis ( a - e ); simple linear regression analysis ( f ); two-way ANOVA with Sidak’s post hoc analysis ( g,h ); ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Expressing, Control, In Vitro, Activation Assay, Incubation, Blocking Assay

Journal: Nature immunology

Article Title: A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma

doi: 10.1038/s41590-020-0777-3

Figure Lengend Snippet: a , b , Flow cytometric analysis, cell frequencies and mean fluorescence intensity (MFI) of Notch1, 2 and 3 expression in peripheral blood T reg cells ( a ) and T eff cells ( b ) of control and asthmatic subjects, the latter segregated for asthma severity (control n=22, M.P n= 15, Mod n= 16. S.P n=11). c , Flow cytometric analysis and cell frequencies of Notch4 peripheral blood T reg cells of healthy control, food allergy (FA), eczema and FA+eczema (Control n=37, FA n= 28, Eczema n=10 and FA+Eczema n=20) d , Serum GDF15 concentrations in asthmatic subjects plotted as a function of Notch4 expression on circulating T reg cells (n=73) e , Cell frequencies of Notch4 expression in peripheral blood T reg cells in healthy subjects, allergic and non-allergic asthmatics (control = 56, non-allergic n=21, allergic n=85). Error bars indicate SEM. Statistical tests: One-way ANOVA with Dunnett’s post hoc analysis. ( a - c,e ); simple regression analysis ( d ). ***P<0.001, ****P<0.0001. Data representative of two or three independent experiments.

Article Snippet: T reg cells at these different concentrations, 1:5, 1:10 or 1:20, from Foxp3 YFPCre mice either sham treated or treated OVA+UFP with high Notch4 expression with or without the addition of anti-Notch4 mAb (clone: HMN4–14, Bioxcell) in concentration of 10ng/ml, T reg cells from Foxp3 YFPCre Notch4 Δ/Δ , Foxp3 YFPCre Ctnnb1 Δ/Δ or Foxp3 YFPCre Yap1 Δ/Δ Wwtr1 Δ/Δ mice treated with OVA+UFP.

Techniques: Fluorescence, Expressing, Control